L Protein Requirement for In Vitro RNA Synthesis by Vesicular Stomatitis Virus

Initiation of RNA Synthesis in Vitro by Vesicular Stomatitis Virus

The ATP requirement during RNA transcription by the RNA-dependent RNA polymerase associated with purified vesicular stomatitis virus was studied during chain initiation and chain elongation steps. Initiation of transcription in vitro has an apparent K,,, for ATP of approximately 500 PM, whereas the apparent Km for ATP during chain elongation is almost identical with those for the other three ri...

Coupled in vitro transcription and translation of vesicular stomatitis virus messenger RNA ( vesicular stomatitis virus ribonucleoprotein cores / virion - associated RNA polymerase / cell - free protein synthesis / slab gel electrophoresis )

The virion transcriptase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) of vesicular stomatitis virus was fully active when ribonucleoprotein cores from purified virions were added to cell-free protein synthesizing systems of eukaryotic origin. Synthesis of mRNA was linear for at least 3 hr and the newly synthesized viral mRNA was efficiently utilized for the synthesis of viral...

Initiation of RNA synthesis in vitro by vesicular stomatitis virus. Role of ATP.

The ATP requirement during RNA transcription by the RNA-dependent RNA polymerase associated with purified vesicular stomatitis virus was studied during chain initiation and chain elongation steps. Initiation of transcription in vitro has an apparent Km for ATP of approximately 500 micron, whereas the apparent Km for ATP during chain elongation is almost identical with those for the other three ...

Rna Complexes in Vesicular Stomatitis Virus Infected L-cells Studies

Vesicular Stomatitis Virus G Protein by Swainsonine

Swainsonine, an inhibitor of glycoprotein processing, inhibits the formation of the normal oligosaccharide chain of the G protein of vesicular stomatitis virus. Thus, when vesicular stomatitis virus was grown in baby hamster kidney cells in the presence of swainsonine (15 to 500 ng/ml) and labeled with [2-3H]mannose, the oligosaccharide portion of the G protein was completely susceptible to the...